Biomedical application of immobilized enzymes
نویسندگان
چکیده
منابع مشابه
Coupled reactions of immobilized enzymes and immobilized substrates: clinical application as exemplified by amylase assay.
We described a partitioned enzyme-sensor system, which incorporates an immoblized substrate and three or more discrete immobilized enzymes. This instrument measures alpha-amylase activity by passing the solution containing alpha-amylase over a column packed with immobilized starch. The resulting oligosaccharides are successively exposed to a column or columns containing immobolized glucose oxid...
متن کاملAnalytical applications of immobilized enzymes.
Several clinical laboratory methods using enzymes as reagents now may utilize immobilized enzymes. These are enzymes attached to solid surfaces by adsorption, covalent binding, cross linking or similar means. Immobilized enzymes are widely used presently in convenient tests for urine glucose and galactose or blood glucose and urea, and in serum glucose or urea determinations by automated method...
متن کاملBiomedical Applications of Immobilized Enzymes and Proteins, Vol. I and II
In the last ten years great advances have been made in elucidating the properties of immobilized enzymes, which have already found practical applications in chemical analysis as well as in large scale industrial processes. The term "immobilized enzymes" embraces a variety of systems in which one or more enzymes are sequestered in gels or microcapsules, covalently bound or adsorbed to solid supp...
متن کاملImmobilized enzymes: crystals or carriers?
The advantages of immobilized over soluble enzymes arise from their enhanced stability and ease of separation from the reaction media, leading to significant savings in enzyme consumption. Immobilization methods range from binding to prefabricated carrier materials to packaging in enzyme crystals or powders. During their use, mass-transfer effects can produce substrate or pH gradients, which re...
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ژورنال
عنوان ژورنال: Journal of Pharmaceutical Sciences
سال: 2000
ISSN: 0022-3549,1520-6017
DOI: 10.1002/1520-6017(200008)89:8<979::aid-jps2>3.0.co;2-h